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The essential oil was subjected to gas chromatography analyses to obtain its chemical profile. alternifolia essential oil by gas chromatography-mass spectrometry (GC-MS) endodontalis.ĭetermination of the chemical profile of M. Thus, the aim of this study was to evaluate the effects of Melaleuca alternifolia oil and chlorhexidine on the viability and VSC production of P. alternifolia, there are few studies evaluating its activity against oral pathogens that cause bad breath Despite the antimicrobial potential of M. A solution containing tea tree oil was shown to reduce the levels of malodour and production of VSCs in patients nursed in an intensive care unit Melaleuca alternifolia, also known as tea tree oil, has been studied because of its antimicrobial activity against oral pathogens, showing inhibitory and bactericidal effects Most products used to reduce malodour have antimicrobial properties, and the decrease in VSCs is usually related to the suppression of bacterial growth. It has also been reported that natural products, such as green tea, produce effects that control halitosis and VSC production Various oral approaches have been employed to treat halitosis, including the mechanical removal of tongue and subgingival biofilms, the use of chlorhexidine, cetylpyridinium, or essential oil mouthrinses, and the application of masking products such as chewing gums and mouthrinses containing chlorine dioxide and zinc salts In addition to the role of VSCs in generating halitosis, there is evidence suggesting that these gases are also involved in the pathogenesis of periodontal diseases Some anaerobic gram-negative bacteria present in the oral cavity, such as Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia, Tannerella forsythia, and Porphyromonas endodontalis, are the main species responsible for the production of VSCs Oral bad breath can result from the degradation of proteins containing sulphur amino acids (methionine and cysteine), resulting in the production of volatile sulphur compounds (VSCs), represented by hydrogen sulfide (H 2S), methyl mercaptan (CH 3SH), and dimethyl sulphide : gases that emanate malodour Periodontal disease, peri-implantitis, deep carious lesions, tongue coating, impacted food or debris, unclean dentures, and other oral problems may contribute to the onset of halitosis This condition is multifactorial and may comprise both oral and non-oral causes The origin of pathological halitosis can be systemic or local , leading to personal and social discomfort Halitosis, also known as bad breath or malodour, is a condition caused by fetid odours present in air emanating from the mouth